Bivalirudin is a short 21 amino acids peptide. It has 2 basic and 6 acidic groups. It is a naturally occurred an anticoagulant. Asp9 – bivalirudin is a process impurity and can be a result of degradation of bivalirudin drug.
| Column | Promix MP, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 30-60%, 35 min |
| Buffer | Na2HPO4 pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 215 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Blood thinners |
| Analyzing Compounds | Bivalirudin |
Application Column
Promix MP
The Promix family of mixed-mode columns presents an innovative chromatography technology for the efficient resolution of peptides and proteins. This technology hinges on a unique blend of hydrophobic and ionic interactions, facilitated by a novel separation medium: a ligand bonded to a silica support, chemically combined with hydrophobic and ionic functional groups. This phase provides unparalleled selectivity and peak capacity. By independently adjusting the quantities of buffer and organic modifier, a virtually infinite number of separation conditions can be achieved, rendering it suitable for a wide array of biomolecules.
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