HPLC Analysis of Bivalirudin on Promix MP colum



Bivalirudin is a short 21 amino acids peptide.  It has 2 basic and 6 acidic groups. It is a naturally occurred  an anticoagulant.  Asp9 – bivalirudin is a process impurity and can be a result of degradation  of bivalirudin drug.



Column Promix MP, 4.6×250 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 30-60%, 35 min
Buffer Na2HPO4 pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 215 nm



Class of Compounds
Acid, Hydrophilic, Ionizable, Blood thinners
Analyzing Compounds Bivalirudin


Application Column

Promix MP

The Promix family of mixed-mode columns presents an innovative chromatography technology for the efficient resolution of peptides and proteins. This technology hinges on a unique blend of hydrophobic and ionic interactions, facilitated by a novel separation medium: a ligand bonded to a silica support, chemically combined with hydrophobic and ionic functional groups. This phase provides unparalleled selectivity and peak capacity. By independently adjusting the quantities of buffer and organic modifier, a virtually infinite number of separation conditions can be achieved, rendering it suitable for a wide array of biomolecules.

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Application Analytes:

Application Detection:
UV Detection
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