HPLC Analysis of Bivalirudin on Promix MP colum

  Chr_200 Bivalirudin is a short 21 amino acids peptide.  It has 2 basic and 6 acidic groups. It is a naturally occurred  an anticoagulant.  Asp9 - bivalirudin is a process impurity and can be a result of degradation  of bivalirudin drug.  


Column Promix MP, 4.6x250 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 30-60%, 35 min
Buffer Na2HPO4 pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 215 nm


Class of Compounds Acid, Hydrophilic, Ionizable, Blood thinners
Analyzing Compounds Bivalirudin
Application Analytes: