Benzidine dihydrochloride

Benzidine dihydrochloride structural formula

CAS Number531-85-1
Molecular FormulaC12H14Cl2N2
Molecular Weight257.161
InChI KeyRUAXWVDEYJEWRY-UHFFFAOYSA-N
LogP1.45
Synonyms
  • Benzidine dihydrochloride
  • [1,1'-Biphenyl]-4,4'-diamine--hydrogen chloride (1/2)
  • [1,1'-Biphenyl]-4,4'-diamine, hydrochloride (1:2)
  • 531-85-1
  • EINECS 208-519-6
  • NSC 3500
  • Benzidine HCl
  • Dihidrocloruro de benzidina
  • UNII-LVU06XHC39

Applications:

Separation of Benzidine dihydrochloride on Newcrom R1 HPLC column

February 19, 2018
Separation of Benzidine dihydrochloride on Newcrom C18 HPLC column

Benzidine dihydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Benzidine dihydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.