alpha-Ketoisocaproic acid

.alpha.-Ketoisocaproic acid structural formula

CAS Number816-66-0
Molecular FormulaC6H10O3
Molecular Weight130.144
InChI KeyBKAJNAXTPSGJCU-UHFFFAOYSA-N
LogP0.531
Synonyms
  • .alpha.-Ketoisocaproic acid
  • 4-Methyl-2-oxopentanoic acid
  • Pentanoic acid, 4-methyl-2-oxo-
  • 816-66-0
  • Pentanoic acid, 4-methyl-2-oxo-
  • 2-keto-4-Methylvaleric acid
  • 2-Ketoisocaproic acid
  • 2-Oxo-4-methylpentanoic acid
  • 2-Oxo-4-methylvaleric acid
  • 2-Oxoisocaproic acid
  • 2-Oxoleucine
  • 4-Methyl-2-oxopentanoic acid
  • 4-Methyl-2-oxovaleriansaure
  • 4-methyl-2-oxovaleric acid
  • Acide 4-methyl-2-oxovalerique
  • acido 4-metil-2-oxovalerico
  • Ketoleucine
  • VALERIC ACID, 4-METHYL-2-OXO-
  • ?-Ketoisocaproic acid
  • ?-Ketoisocapronic acid
  • ?-Oxoisocaproic acid
  • EINECS 212-435-5
  • UNII-4GUJ8AH400
  • 2-Keto-4-Methylvalerate
  • 2-Ketoisocaproate
  • 2-Oxo-4-methylpentanoate
  • 2-Oxo-4-methylvalerate
  • 2-Oxo-4-methylvaleric acid
  • 2-keto-4-methyl-valeric acid
  • 4-Methyl-2-oxo-Valerate
  • 4-Methyl-2-oxo-Valeric acid
  • 4-Methyl-2-oxopentanoate
  • 4-methyl-2-oxo-pentanoic acid
  • 4-methyl-2-oxopentanoic acid
  • Ketoisocaproate
  • Ketoisocaproic acid
  • Methyloxovalerate
  • Methyloxovaleric acid
  • Oxoisocaproate
  • Oxoisocaproic acid
  • a-Ketoisocaproate
  • a-Ketoisocaproic acid
  • a-Ketoisocapronate
  • a-Ketoisocapronic acid
  • a-Oxoisocaproate
  • a-Oxoisocaproic acid
  • alpha-Keto-isocaproate
  • alpha-Keto-isocaproic acid
  • alpha-Ketoisocaproate
  • alpha-Ketoisocapronate
  • alpha-Ketoisocapronic acid
  • alpha-Oxoisocaproate
  • alpha-Oxoisocaproic acid
  • alpha-ketoisocaproic acid

Applications:

Uv-Vis Spectrum of α-Ketoisocaproic acid

March 3, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze alpha-Ketoisocaproic acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in an acetonitrile and water 50/50 mobile phase.

 

Application Analytes:
alpha-Ketoisocaproic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of .alpha.-Ketoisocaproic acid on Newcrom R1 HPLC column

February 19, 2018
Separation of .alpha.-Ketoisocaproic acid on Newcrom C18 HPLC column

.alpha.-Ketoisocaproic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
alpha-Ketoisocaproic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.