Acridine Orange

Acridine Orange structural formula

CAS Number494-38-2
Molecular FormulaC17H19N3
Molecular Weight265.360
InChI KeyDPKHZNPWBDQZCN-UHFFFAOYSA-N
LogP3.67
Synonyms
  • Acridine Orange
  • N3,N3,N6,N6-Tetramethylacridine-3,6-diamine
  • 3,6-Acridinediamine, N3,N3,N6,N6-tetramethyl-
  • 494-38-2
  • 4-22-00-05490
  • C.I. No. 46005:1
  • Acridine Orange Free Base
  • 2,8-Bisdimethylaminoacridine
  • 3,6-Bis(dimethylamino)acridine
  • BRN 0020916
  • NSC 194350
  • N,N,N(sup 1),N(sup 1)-Tetramethylacridinediamine
  • N,N,N',N'-Tetramethylacridinediamine
  • N,N,N',N'-Tetramethyl-3,6-acridinediamine
  • Waxoline Orange A
  • 3,6-Bis-(dimethylamino)akridin
  • Oranz akridinova
  • Oranz rozpoustedlova 15
  • UNII-F30N4O6XVV
  • 3,6-Di(dimethylamino)acridine
  • Acridine Orange Base
  • Brilliant Acridine Orange E
  • C.I. 46005B
  • C.I. Solvent Orange 15
  • N,N,N',N'-Tetramethyl-3,6-acridinediamine
  • Solvent Orange 15

Applications:

Separation of Acridine Orange on Newcrom R1 HPLC column

May 16, 2018
Separation of Acridine Orange on Newcrom R1 HPLC column

Acridine Orange can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Acridine Orange
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.