Acid Blue 25 parent

Acid Blue 25 parent structural formula

CAS Number2786-71-2
Molecular FormulaC20H14N2O5S
Molecular Weight394.400
InChI KeyOPBOOIFXQHPAPJ-UHFFFAOYSA-N
LogP1.75
Synonyms
  • Acid Blue 25 parent
  • 1-Amino-4-anilino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
  • 2-Anthracenesulfonic acid, 1-amino-9,10-dihydro-9,10-dioxo-4-(phenylamino)-
  • 2786-71-2
  • C.I. Acid Blue 25 free acid
  • 1-Amino-4-anilino-2-anthraquinonesulfonic acid
  • EINECS 220-508-8
  • NSC 167429
  • 1007631-92-6

Applications:

Separation of Acid Blue 25 parent on Newcrom R1 HPLC column

May 16, 2018
Separation of Acid Blue 25 parent on Newcrom R1 HPLC column

Acid Blue 25 parent can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Acid Blue 25 parent
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.