Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270nm.
|Column||Primesep 200, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 30/70%|
|Buffer||AmFm pH 3.0- 30 mM|
|Flow Rate||1.0 ml/min|
|Detection||UV, 270 nm|
|Class of Compounds||Drug, Acid, Hydrophilic, Ionizable,Neurotransmitter|
|Analyzing Compounds||Octopamine, Synephrine|