Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270nm.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable,Neurotransmitter |
| Analyzing Compounds | Octopamine, Synephrine |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSynephrine
