Tetramethylammonium

CAS Number51-92-3
Molecular FormulaC4H12N
Molecular Weight74.146
InChI KeyQEMXHQIAXOOASZ-UHFFFAOYSA-N
LogP-2.41
Synonyms
  • Tetramethylammonium
  • N,N,N-Trimethylmethanaminium
  • Methanaminium, N,N,N-trimethyl-
  • 51-92-3

Applications:

HPLC Method for Analysis of Tetramethylammonium on Newcrom AH Column

July 11, 2023

HPLC Method for Analysis of Tetramethylammonium on (Column not found) by SIELC Technologies

HPLC Method for Analysis of Tetramethylammonium on (Column not found) by SIELC Technologies

Tetramethylammonium is a quaternary ammonium cation with the formula (CH3)4N+. This compound is one of the simplest quaternary ammonium ions, and is notable for its prevalence in both science and industry.

The central nitrogen atom in tetramethylammonium has a positive charge due to the four alkyl (methyl, CH3) groups attached to it. This makes it a cation, meaning it carries a positive charge.

This compound is often used in its chloride salt form (tetramethylammonium chloride), which is a white crystalline powder that is highly soluble in water and used in various chemical applications, such as phase transfer catalysts, surfactants, and reagents in organic synthesis.

Tetramethylammonium ions can also serve as counter ions or blocking ions in certain biological and chemical studies. For example, they are sometimes used to block potassium channels in studies of cellular physiology.

The tetramethylammonium can be retained, analyzed, on a Newcrom AH column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tetramethylammonium

Condition

Column(Column variation not found)
Mobile PhaseMeCN/H2O – 50/50%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 50 °C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
(MS- compatible mobile phase)
Peak Retention Time5.12 min
Sample concentration1 mg/ml
Injection volume1 µl
LOD 320 ppb

Description

Class of CompoundsHydrophilic, Metal, Ion, Quaternary ammonium salt
Analyzing CompoundsTetramethylammonium
Application Analytes:
Tetramethylammonium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Sodium, Potassium Ions and Tetramethylammonium Chloride on Newcrom AH Column

September 8, 2020

HPLC Method for Analysis of Tetramethylammonium, Sodium, Potassium on Newcrom AH by SIELC Technologies


Tetramethylammonium chloride is a quaternary ammonium salt used widely as a reagent in industrial applications.  It can be separated from sodium and potassium chlorides on a mixed-mode Newcrom AH column with a simple isocratic MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

ColumnNewcrom AH, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 50/50%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionCAD (Corona) MS- compatible mobile phase
Class of CompoundsHydrophilic, Metal, Ion, Quaternary ammonium salt
Analyzing CompoundsTetramethylammonium, Sodium, Potassium

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Potassium
Sodium
Tetramethylammonium

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column

July 7, 2011

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Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.

Condition

Column Primesep 200,  4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Ions,  Hydrophilic, Ionizable, Quaternary amines
Analyzing Compounds Sodium, Chloride, Tetramethylammonium,  Tetrabutylammonium

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Sodium
Tetrabutylammonium
Tetramethylammonium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Quaternary Amines

September 11, 2003

Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).

Condition

Column Primesep C, 4.6×50 mm
Mobile Phase MeCN/H2O
Buffer TEA acetate
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Quaternary Amines, Ionizable, Hormone
Analyzing Compounds Methyltriethylammonium, tetraethylammonium, Tetramethylammonium

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromide
Methyltriethylammonium Bromide
Quaternary Amines
Tetraethylammonium
Tetramethylammonium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.