Separation of Quaternary Amines

Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).

Condition
Column Primesep C, 4.6×50 mm
Mobile Phase MeCN/H2O
Buffer TEA acetate
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Quaternary Amines, Ionizable, Hormone
Analyzing Compounds Methyltriethylammonium, tetraethylammonium, Tetramethylammonium

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromide
Methyltriethylammonium Bromide
Quaternary Amines
Tetraethylammonium
Tetramethylammonium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.