Separation of Quaternary Amines

Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).


Column Primesep C, 4.6x50 mm
Mobile Phase MeCN/H2O
Buffer TEA acetate
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Quaternary Amines, Ionizable, Hormone
Analyzing Compounds Methyltriethylammonium, tetraethylammonium, Tetramethylammonium
Application Analytes:
Methyltriethylammonium Bromide
Quaternary Amines