Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column

chr_300.gif chr_301.gif Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.

Condition

Column Primesep 200,  4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH4.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable, Quaternary amines
Analyzing Compounds Sodium, Chloride, Tetramethylammonium,  Tetrabutylammonium
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Sodium
Tetrabutylammonium
Tetramethylammonium

Application Detection:
ELSD/MS Detection