Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column

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Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.

Condition
Column Primesep 200,  4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Ions,  Hydrophilic, Ionizable, Quaternary amines
Analyzing Compounds Sodium, Chloride, Tetramethylammonium,  Tetrabutylammonium

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sodium
Tetrabutylammonium
Tetramethylammonium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.