| CAS Number | 343-65-7 |
|---|---|
| Molecular Formula | C10H12N2O3 |
| Molecular Weight | 208.217 |
| InChI Key | YGPSJZOEDVAXAB-UHFFFAOYSA-N |
| LogP | -2.2 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Kynurenine on Primesep 100 Column
August 8, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenine
Kynurenine is a key metabolite in the tryptophan catabolic pathway, often referred to as the kynurenine pathway. This pathway is responsible for breaking down the essential amino acid tryptophan into several bioactive compounds.
Kynurenine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Kynurenine and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 35% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Samples | 0.5 mg/ml in H2O – 100% |
| Injection volume | 1 µl |
| LOD* | 20 ppb (250 nm) |
| Class of Compounds | Kynurenines |
| Analyzing Compounds | Kynurenine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV-Vis Spectrum of Kynurenine
June 28, 2024
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Kynurenine. Absorption Maxima: 228 nm, 256 nm, 368 nm.
If you are looking for optimized HPLC method to analyze Kynurenine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
HPLC Method for Separation of a Mixture of Tryptophan and its Catabolites on Primesep 100 Column
October 3, 2023
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mixture of Tryptophan and its Catabolites on Primesep 100 by SIELC Technologies
Tryptophan and its catabolites participate in several biological pathways, having roles in protein synthesis, serving as precursors to bioactive molecules, and influencing several physiological processes.
Tryptophan is an essential amino acid with the chemical formula C11H12N2O2. It cannot be produced within the body and must be obtained through consuming it. Like the other essential proteins, it is a building block for protein and muscle tissue, but it is also converted in the body into serotonin, which affects mood. L-Tryptophan is also used in treatments for severe PMS symptoms, depression, and insomnia. It is naturally found in red meat, poultry eggs, and dairy. You can find detailed UV spectra of Tryptophan and information about its various lambda maxima by visiting the following link.
3-Hydroxyanthranilic acid is an intermediate in the metabolism of tryptophan with the chemical formula C7H7NO3. It is used primarily to prevent the autoxidation of soybean oil and powder. You can find detailed UV spectra of 3-Hydroxyanthranilic acid and information about its various lambda maxima by visiting the following link.
3-Hydroxykynurenine is a metabolite of tryptophan with the chemical formula C10H12N2O4. It can generate reactive oxygen species, potentially contributing to cellular stress and is one of the two known pigments responsible for the yellow coloration of goldenrod crab spiders.
Kynurenine is a metabolite of L-tryptophan with the chemical formula C10H12N2O3. It is primarily used in the production of niacin. It protects the eye by absorbing UV light alongside 3-hydroxykynurenine.
Kynurenine is a key metabolite in the tryptophan catabolic pathway, often referred to as the kynurenine pathway. This pathway is responsible for breaking down the essential amino acid tryptophan into several bioactive compounds. You can find detailed UV spectra of Kynurenine and information about its various lambda maxima by visiting the following link.
Picolinic Acid is a derivative of pyridine with the chemical formula C6H5NO2. It’s function remains a mystery to this day, but it is suspected to have a wide variety of neuroprotective, immunological, and anti-proliferative effects. You can find detailed UV spectra of Picolinic Acid and information about its various lambda maxima by visiting the following link.
Tryptophan, Picolinic Acid, Kynurenine, 3-Hydroxykynurenine, 3-Hydroxyanthranilic acid can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 220 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN 5-50% in 10 min, with 2 min hold afterwards |
| Buffer | Gradient H2SO4 from 0.1-0.2% in 10 min, with 2 min hold afterwards |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
Description
| Class of Compounds | Essential Amino Acid Tryptophan and its Catabolites |
| Analyzing Compounds | Tryptophan, Picolinic Acid, Kynurenine, 3-Hydroxykynurenine, 3-Hydroxyanthranilic acid |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
3-Hydroxykynurenine
Kynurenine
Picolinic Acid
Tryptophan
HPLC Analysis of Kynurenine
July 31, 2015
Kynurenine is an important biological compound which carries out a number of biological functions including dilating blood vessles and regulating immune response. Kynurenine was retained on Legacy L1 which containes embedded C18 groups. Comparisons between this chromatagram and from Phenomenex columns are available upon request.
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 0-80%, 20 min |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 225 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Kynurenine |
&
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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