HPLC Method for Analysis of Kynurenine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Kynurenine on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenine

Kynurenine is a key metabolite in the tryptophan catabolic pathway, often referred to as the kynurenine pathway. This pathway is responsible for breaking down the essential amino acid tryptophan into several bioactive compounds.

Kynurenine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Kynurenine and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 35%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Samples0.5 mg/ml in H2O – 100%
Injection volume1 µl
LOD*20 ppb (250 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Kynurenines
Analyzing CompoundsKynurenine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Kynurenine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.