Crystal Violet Lactone

Crystal Violet Lactone structural formula

CAS Number1552-42-7
Molecular FormulaC26H29N3O2
Molecular Weight415.537
InChI KeyIPAJDLMMTVZVPP-UHFFFAOYSA-N
LogP4.24
Synonyms
  • Crystal Violet lactone
  • 6-(Dimethylamino)-3,3-bis[4-(dimethylamino)phenyl]-2-benzofuran-1(3H)-one
  • 1(3H)-Isobenzofuranone, 6-(dimethylamino)-3,3-bis[4-(dimethylamino)phenyl]-
  • 1552-42-7
  • 1(3H)-Isobenzofuranone, 6-(dimethylamino)-3,3-bis[4-(dimethylamino)phenyl]-
  • 3,3-Bis (p-dimethylaminophenyl)-6-dimethylaminophthalide
  • 3,3-Bis(4-dimethylaminophenyl)-6-dimethylaminophthalide
  • 3,3-Bis(p-dimethylaminophenyl)-6-dimethylaminophthalate
  • 3,3-Bis(p-dimethylaminophenyl)-6-dimethylaminophthalide
  • 3,3-Bis[4-(dimethylamino)phenyl]-6-(dimethylamino)-1,3-dihydroisobenzofuran-1-one
  • 3,3-Bis[4-(dimethylamino)phenyl]-6-(dimethylamino)phthalide
  • 3,3-Bis[p-(dimethylamino)phenyl]-6-(dimethylamino)phthalide
  • 6-(Dimethylamino)-3,3-bis(4-(dimethylamino)phenyl)phthalide
  • 6-(Dimethylamino)-3,3-bis[4-(dimethylamino)phenyl]-1(3H)-isobenzofuranone
  • 6-DIMETHYLAMINO-3,3-BIS(4-(DIMETHYLAMINO)PHENYL)PHTHALIDE
  • 6-Dimethylamino-3,3-bis(4-dimethylaminophenyl)phtalide
  • 6-Dimethylamino-3,3-bis(4-dimethylaminophenyl)phthalid
  • 6-dimethylamino-3,3-bis(4-dimethylaminophenyl)phthalide
  • 6-dimetilamino-3,3-bis(4-dimetilaminofenil)ftalida
  • Color Former CVL
  • Copikem I
  • ISOBENZOFURAN(3H)-1-ONE, 6-(DIMETHYLAMINO)-3,3-BIS[4-(DIMETHYLAMINO)PHENYL]-
  • NSC 32991
  • NSC 3562
  • Phthalide, 6-(dimethylamino)-3,3-bis[p-(dimethylamino)phenyl]-
  • EINECS 216-293-5
  • Phthalide, 6-(dimethylamino)-3,3-bis(p-(dimethylamino)phenyl)-
  • UNII-USS7CW0676
  • 129652-58-0

Applications:

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column

December 7, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column
HPLC Method for Analysis of Pararosaniline, Crystal Violet and Crystal Violet Lactone on Primesep 100 Column by SIELC Technologies

Pararosaniline (Basic Red 9) is a popular basic magenta dye and part of the triarylmethane family of dyes. Crystal Violet (Methyl Violet 10B), another basic triarylmethane dye, is frequently used for histological stains and for identifying Gram-positive bacteria. Crystal Violet Lactone, a derivative of Crystal Violet, is a basic thermochromic dye widely used as a security marker for various types of fuels. These three basic dyes can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline, Crystal Violet and Crystal Violet Lactone

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 80/20%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 540, 590, 200 nm
Peak Retention Time3.15, 7.24, 8,82 min

Description

Class of CompoundsDyes
Analyzing CompoundsPararosaniline, Crystal Violet and Crystal Violet Lactone

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Crystal Violet
Crystal Violet Lactone
Pararosaniline Hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Dyes

November 21, 2010


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Condition 1
Column Newcrom B, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 


Various dyes are widely used in food, textile, and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone, and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.

Condition  2
Column Primesep D, 4.6×50 mm, 3 µm, 100A
Mobile Phase MeCN
Buffer H3PO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 300 nm

 

Description
Class of Compounds
 Dye, Hydrophilic, Ionizable
Analyzing Compounds Methylene Blue, Crystal violet lactone, Crystal violet

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Crystal Violet
Crystal Violet Lactone
Methylene Blue
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.