Carboxin or carboxine is an oxathin fungicide considered by the EURL (European Union Reference Laboratory) to be a target pesticide. It was placed on a Target Pesticide List for the EUPT-CF9 2015 (European Union Proficiency Test for Cereals and Feeding stuff). A method for separating carboxin was developed for Primesep 100 and Obelisc R columns. Both columns are mixed-mode offering unique retention tuning. Obelisc R contains ion-pairing groups and a long hydrophobic chain, while Primesep 100 contains embedded acidic ion-pairing groups. Method is LC/MS compatible and can be used on many pesticides and herbicides.
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 25-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 30-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Obelisc R, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Primesep 100, 2.1x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Fungicide Carboxin |
Application Analytes:
Carboxin