Column | Primesep B2, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
Flow Rate | 0.4 ml/min |
Detection | UV, 250 nm |
Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
Flow Rate | 0.4 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
Analyzing Compounds | Phosphamidon |
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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