Monocrotophos Separation on Obelisc and Primesep Mixed-Mode HPLC Columns

 

Banned in the U.S. and many other countries, Monocrotophos is a cheap and toxic organophosphate insecticide. It is especially toxic to birds, being blamed for widespread bird kills. The EURL (European Reference Laboratory) included monocrotophos in a the EURL-FV a proficency test for multi-residue methods using QuEChERS (Quick, East, Cheap, Effective, Rugged, Safe) followed bby LC/MS/MS for fruits and vegitables. Primesep B2 and Obelisc R were used to retain monocrotophos and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing monocrotophos, other organophosphate insecticides, and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Monocrotophos
 
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Application Analytes:
Monocrotophos

Application Detection:
UV Detection