Captan is a fungicide that is typically combined with other pesticides. It is in the class of fungicides called phthalimide, and is used on a number of vegetables and ornamental plants. Captan reduces infections on the surface of plant material improving their appearance. The EURL-SRM (European Union Reference Laboratory – Single Residue Methods) included captan in a list of pesticides difficult to analyze by traditional multiresidue methods. We separated and analyzed captan using two mixed-mode columns with different modes of separation. Obelisc R has a long hydrophobic chain and multiple ion-pairing groups, and Primesep 100 contains acidic ion-pairing groups. Method is LC/MS compatible and can be use on many different pesticides.
Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 25-70% |
Buffer | Gradient AmAc pH 3.0- 30-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70% |
Buffer | Gradient AmAc pH 3.0- 20-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Column | Primesep 100, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70% |
Buffer | Gradient AmAc pH 3.0- 20-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Insecticide, Pesticide, Hydrophobic, Ionizable |
Analyzing Compounds | Captan |
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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