HPLC Separation of Captan on Obelisc R and Primesep 100 Columns

   

Captan is a fungicide that is typically combined with other pesticides. It is in the class of fungicides called phthalimide, and is used on a number of vegetables and ornamental plants. Captan reduces infections on the surface of plant material improving their appearance. The EURL-SRM (European Union Reference Laboratory - Single Residue Methods) included captan in a list of pesticides difficult to analyze by traditional multiresidue methods. We separated and analyzed captan using two mixed-mode columns with different modes of separation. Obelisc R has a long hydrophobic chain and multiple ion-pairing groups, and Primesep 100 contains acidic ion-pairing groups. Method is LC/MS compatible and can be use on many different pesticides.

 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%
Buffer Gradient AmAc pH 3.0- 30-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Pesticide, Hydrophobic, Ionizable
Analyzing Compounds Captan
 
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Application Analytes:
Captan

Application Detection:
UV Detection