Dichloran (DCNA) is a fungicide analyzed on the mixed-mode columns Obelisc R and Primesep 100. DCNA was separated from impurities on Obelisc R, which contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Primesep 100 which contains embedded acidic ion-pairing groups also retained DCNA. Method is LC/MS compatible and can be used as a general approach for analyzing DCNA and many other pesticides.
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 25-70% |
| Buffer | Gradient AmAc pH 3.0- 30-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70% |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Primesep 100, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70% |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Insecticide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dichloran |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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