Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns

4-(2,4-dichlorophenoxy)butyric acid or 2,4-DB is a herbicides used in alfalfa, peanuts, and soybean production. 2,4-DB selectively controls broadleaf weeds inhibiting growth at the root and stem tips. It is considered slightly toxic, as is its metabolite 2,4-D. The EURL (European Union Reference Laboratory) included 2,4-DB in an analysis of acidic pesticides using QuEChERS methodology. Primesep 100 and Obelisc R were used to retain and separate 2,4-DB from impurities. Method is LC/MS compatible and developed to be used for many pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4-DB
 
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Application Analytes:
2,4-DB

Application Detection:
UV Detection