Analysis of Naled on Primesep and Obelisc Mixed-Mode HPLC Columns

 

Naled is an organophosphate insecticide that shows relatively low toxicity. While home use has been discontinued, naled is still used in public mosquito eradication programs. Naled can be reduced to dichlorvos, which the EURL (European Union Reference Laboratory), included as a target pesticide for the EUPT-CF9 a proficiency test for cereals and feedingstuff that require muli-residue methods. Primesep B2 and Obelisc R were used to retain naled and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing naled, other organophosphate insecticides, and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Naled
 
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Application Analytes:
Naled

Application Detection:
UV Detection