5-Methoxyindoleacetic acid

5-Methoxyindoleacetic acid structural formula

CAS Number3471-31-6
Molecular FormulaC11H11NO3
Molecular Weight205.213
  • 5-Methoxyindoleacetic acid
  • (5-Methoxy-1H-indol-3-yl)acetic acid
  • 1H-Indole-3-acetic acid, 5-methoxy-
  • 3471-31-6
  • EINECS 222-438-3
  • Methoxyindoleacetic acid
  • 5-Methoxyindole-3-acetic acid


Separation of 5-Methoxyindoleacetic acid on Newcrom R1 HPLC column

May 16, 2018
Separation of 5-Methoxyindoleacetic acid on Newcrom R1 HPLC column

5-Methoxyindoleacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
5-Methoxyindoleacetic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.