Chromatography Webinar Series 2025

🔹 Session 1: Fundamentals of Chromatography – Principles & Techniques

• Overview of chromatography types
• Key concepts: retention, selectivity, efficiency, resolution
• Common applications in pharmaceuticals, biotech, food, and environmental sciences

🔹 Session 2: Mastering HPLC – Fundamentals, Method Development & Optimization

• Core components of HPLC: pumps, columns, detectors, and mobile phases
• Choosing the right column and mobile phase for different analytes
• Gradient vs. isocratic elution and their impact on separation
• Factors affecting resolution, efficiency, and sensitivity

🔹 Session 3: Introduction to Mixed-Mode Chromatography in HPLC

• What is mixed-mode chromatography?
• Advantages over single-mode techniques (RP, IEX, HILIC, etc.)
• Mechanisms of multiple interactions: hydrophobic, ionic, hydrogen bonding, and π-π interactions
• How mixed-mode selectivity can improve complex separations

🔹 Session 4: Practical Method Development in Mixed-Mode Chromatography

• Selecting the right stationary and mobile phases
• Optimization strategies: balancing retention, peak shape, and resolution
• Overcoming common challenges in method development
• Case studies: real-world applications in pharmaceuticals, biological samples, and complex matrices

🔹 Session 5: Real-World Applications & Future Innovations in Mixed-Mode HPLC

• Advanced applications in pharmaceuticals, biotechnology, and food analysis
• Strategies for handling complex sample matrices with mixed-mode chromatography
• Emerging research and novel stationary phases
• Future trends and developments in mixed-mode HPLC