Vanillin

CAS Number121-33-5
Molecular FormulaC8H8O3
Molecular Weight152.15
LogP1.2
Synonyms
  • vanillin
  • 4-Hydroxy-3-methoxybenzaldehyde
  • 121-33-5
  • Vanillaldehyde
  • Vanillic aldehyde
  • p-Vanillin
  • Vanilla
  • Lioxin
  • 2-Methoxy-4-formylphenol
  • Vanilline
  • 4-Hydroxy-m-anisaldehyde
  • 3-Methoxy-4-hydroxybenzaldehyde
  • Zimco
  • Benzaldehyde, 4-hydroxy-3-methoxy-
  • 4-Hydroxy-3-methoxy-benzaldehyde
  • p-Hydroxy-m-methoxybenzaldehyde
  • Vanilin
  • 4-Formyl-2-methoxyphenol
  • Methylprotocatechuic aldehyde
  • Vanillin (natural)
  • 4-Hydroxy-5-methoxybenzaldehyde
  • m-Anisaldehyde, 4-hydroxy-
  • Protocatechualdehyde, methyl-
  • Vanillin (NF)
  • Vanillin [USAN]
  • Protocatechualdehyde 3-methyl ether
  • vaniline
  • Vanillinum
  • Oleoresin vanilla
  • Vanilla oleoresin

Applications:

HPLC Method for Analysis of Vanillin on Primesep SB Column on Alltesta™

September 23, 2025

HPLC Method for Vanillin on Primesep SB by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin

Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.

Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium Formate
Flow Rate1.0 ml/min
DetectionUV 280 nm

Class of CompoundsOrganic Compound
Analyzing CompoundsVanillin

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Vanillin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Vanillin on Primesep SB Column on Cromite™

September 23, 2025

HPLC Method for Vanillin on Primesep SB by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin

Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.

Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnPrimesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium Formate
Flow Rate0.5 ml/min
DetectionUV 280 nm

Class of CompoundsOrganic Compound
Analyzing CompoundsVanillin

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Vanillin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Vanillin on Primesep SB Column

October 15, 2019

 HPLC Method for Vanillin on Primesep SB by SIELC Technologies

  HPLC Determination of Vanillin on Primesep SB Column_1188 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin.

Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.

Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

Condition

Column Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm

 

Description

Class of Compounds Flavoring agent, Hydrophobic, Ionizable
Analyzing Compounds Vanillin

 

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Vanillin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.