| CAS Number | 121-33-5 |
|---|---|
| Molecular Formula | C8H8O3 |
| Molecular Weight | 152.15 |
| LogP | 1.2 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Vanillin on Primesep SB Column on Alltesta™
September 23, 2025
HPLC Method for Vanillin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin
Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.
Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Vanillin |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Method for Analysis of Vanillin on Primesep SB Column on Cromite™
September 23, 2025
HPLC Method for Vanillin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin
Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.
Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium Formate |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Vanillin |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Determination of Vanillin on Primesep SB Column
October 15, 2019
HPLC Method for Vanillin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin.
Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.
Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Flavoring agent, Hydrophobic, Ionizable |
| Analyzing Compounds | Vanillin |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
