HPLC Method for Analysis of Vanillin on Primesep SB Column on Alltesta™

HPLC Method for Vanillin on Primesep SB by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillin

Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.

Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium Formate
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsOrganic Compound
Analyzing CompoundsVanillin

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vanillin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.