HPLC Separation of Polar Pesticides on Newcrom B Column

HPLC Method for Glyphosate, Phosphorous acid, Fosetyl-Al, Bromide, Chlorate, Perchlorate, Bialaphos, Aminomethylphosphonic acid (AMPA), Bromate, Sodium Bromate, 3-(Methylphosphinico)propionic acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate, Phosphorous acid, Fosetyl-Al, Bromide, Chlorate, Perchlorate, Bialaphos, Aminomethylphosphonic acid (AMPA), Bromate, Sodium Bromate, 3-(Methylphosphinico)propionic acid.

Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom B mixed-mode column.

Condition

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 60 mM 15 min
Flow Rate 1 ml/min
Detection CAD

 

Condition 2

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 15 mM 10 min
Flow Rate 1 ml/min
Detection CAD

 

 

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Glyphosate, Phosphorous acid, Fosetyl-Al, Bromide, Chlorate, Perchlorate, Bialaphos, Aminomethylphosphonic acid (AMPA), Bromate, Sodium Bromate, 3-(Methylphosphinico)propionic acid

 

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
3-(Methylphosphinico)propionic acid
Aminomethylphosphonic acid (AMPA)
Bialaphos
Bromate
Bromide
Chlorate
Fosetyl-Al
Glyphosate
Perchlorate
Phosphorous acid
Sodium Bromate

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.