HPLC MS Method for Determination of Malathion Newcrom BH Column

HPLC Method for Analysis of Malathion on Newcrom BH Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Malathion.

Malathion is the most widely used organophosphate pesticide with the chemical formula C10H19O6PS2. In the US, it is used heavily in residential landscaping and in public recreation areas, especially for fruit fly and mosquito eradication. While not considered toxic to humans, malathion metabolizes to a far more toxic malaoxon. The EURL (European Union Reference Laboratory), included malathion as a target pesticide for the EUPT-CF9 a proficiency test for cereals and feedingstuff that require muli-residue methods. can find detailed UV spectra of Malathion and information about its various lambda maxima by visiting the following link.

Malathion can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using LC MS.

Condition

ColumnNewcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferAmFm pH 3.0 – 20 mM
Flow Rate0.5 ml/min
DetectionLC MS ESI SIM 331 [M+H]+

Description

Class of CompoundsPesticide
Analyzing CompoundsMalathion

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Malathion
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.