HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column

HPLC Method for Diclofenac, Carbamazepine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac, Carbamazepine

Diclofenac is an organic compound with the molecular formula C14H11Cl2NO2.

Properties:
Appearance: Typically an odorless, white to off-white crystalline, slightly hygroscopic powder.

Molecular weight: ~296.1 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses:  A nonsteroidal anti-inflammatory drug (NSAID) used to relieve pain and reduce inflammation and swelling.

Carbamazepine is an organic compound with the molecular formula C15H12N2O.

Properties:
Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Diclofenac, Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 220 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 45%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 220 nm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsDiclofenac, Carbamazepine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carbamazepine
Diclofenac
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.