HPLC MS Method for the Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Primesep 200  Column

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole on Primesep 200 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole

It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:

trans-Zeatin:

  • A type of cytokinin.
  • Naturally occurring in plants and plays a key role in promoting cell division and growth.

Kinetin:

  • A synthetic cytokinin.
  • Used in plant tissue culture to stimulate cell division.

N6-(Δ2-Isopentenyl)adenine (iP):

  • Another cytokinin.
  • Found in plants and is involved in regulating growth, development, and differentiation.

N6-Benzyladenine (BA):

  • A synthetic cytokinin.
  • Commonly used in agricultural practices to enhance plant growth and delay senescence.

Brassinazole:

  • Not a cytokinin, but a brassinosteroid biosynthesis inhibitor.
  • Used to study the role of brassinosteroids (another class of phytohormones) in plants.

Thidiazuron (TDZ):

  • A phenylurea derivative that acts as a cytokinin-like compound.
  • Widely used in plant tissue culture to promote shoot regeneration and induce cytokinin-like effects.

trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole can be retained, separated and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 290, 265 nm.

You can find detailed UV spectra of trans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 20%, 4 min than Gradient MeCN/H2O – 20-90% 16 min
BufferFormic Acid -0.2%
Flow Rate0.2 mg/ml
DetectionUV 290 nm, 265 nm ESI SIM 220+, 216+, 204+,226+, 328+, 221+

Class of Compounds
Phytohormone
Analyzing Compoundstrans-Zeatin, Kinetin, N6-(Δ2-Isopentenyl)adenine, Thidiazuron, 6-Benzylaminopurine, Brassinazole

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
6-Benzylaminopurine
Brassinazole
Kinetin
N6-(Δ2-Isopentenyl)adenine
Thidiazuron
trans-Zeatin
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.