Separation type: Liquid Chromatography Reverse Phase
High Performance Liquid Chromatography (HPLC) Method for Analysis of Estragole
Estragole can be retained in HPLC on Newcrom R1 reverse-phase column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Sulfuric Acid buffer. Detection can be achieved by UV detected at 275 nm.
| Column | Newcrom R1, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Phenylpropene |
| Analyzing Compounds | Estragole |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsApplication Analytes:
Estragole
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
