HPLC Chiral Separation of Fipronil Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Fipronil on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fipronil.

Fipronil is an insecticide with the chemical formula C12H4Cl2F6N4OS. It works through causing hyperexcitation of the nerves and muscles in contaminated insects.  In Texas, it is often used to control invasive species of ants and wasps. It is also an active ingredient in flea and tick treatments for pets. WHO classifies it as a Class II, moderately hazardous, pesticide.

You can find detailed UV spectra of Fipronil and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Fipronil enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Fipronil can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA – 85/14/1%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution3.83

Description

Class of CompoundsInsecticide
Analyzing CompoundsFipronil

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fipronil
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.