HPLC Method for Determination of Flunixin and Meglumine on Primesep 100 Column

HPLC Method for Determination of Flunixin and Meglumine on Primesep 100

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Determination of Flunixin Meglumine on Primesep 100 Column by SIELC Technologies
HPLC Method for Determination of Flunixin Meglumine on Primesep 100 by SIELC Technologies

Flunixin is an analgesic and antypyretic nonsteroidal anti-inflammatory drug (NSAID) typically used by veterinarians in bovine and equine treatments. It is typically produced as a Meglumine salt, which helps stabilize and bulk up the medication in its solid form. Flunixin and Meglumine can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 252 nm (Flunixin) with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD (Flunixin and Melumine).

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 60%
Buffer TFA – 0.2%
Flow Rate 1.0 ml/min
Detection ELSD, 50C, UV 252 nm

 

Description

Class of Compounds Drug
Analyzing Compounds Flunixin Meglumine

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Flunixin meglumine
Meglumine (N-Methyl-D-glucamine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.