HPLC Chiral Separation of Vinclozolin on Cyralis CM Column

HPLC Method for Analysis of Vinclozolin on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vinclozolin.

Vinclozolin is a dicarboximide fungicide with the chemical formula C12H9Cl2NO3. It is primarily used to control blights, rots. and molds on fruits, vegetables, and turf. It is considered a possible human carcinogen. It breaks down easily in the environment with the help of microbes between 34 and 94 days, though the half-life residue can reach up to 1000 days. You can find detailed UV spectra of Vinclozolin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Vinclozolin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Vinclozolin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and IPA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA- 90/10%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution2.48

Description

Class of CompoundsFungicide
Analyzing CompoundsVinclozolin

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vinclozolin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.