HPLC Method for Analysis of Troger’s Base on Primesep 200  Column

HPLC Method for Analysis of Troger's base on Primesep 200 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Troger's base.

Troger’s base, also written as Tröger’s base, is a tetracyclic organic compound with the chemical formula C17H18N2. The correct structure for the compound was a mystery for 48 years after it’s initial synthesis by Julius Tröger. You can find detailed UV spectra of Troger's base and information about its various lambda maxima by visiting the following link.

Troger's base can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using LC MS.

Condition

ColumnPrimesep 200, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4  – 0.1%
Flow Rate1.0 ml/min
DetectionUV 200
Limit Of Detection*80 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsOrganic Acid
Analyzing CompoundsTroger's base

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Troger’s base
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.