HPLC Chiral Separation of Praziquantel on Cyralis CM Column

HPLC Method for Analysis of Praziquantel on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Praziquantel.

Praziquantel is an drug with the chemical formula C19H24N2O2It is primarily used to treat parasitic worm infections in a variety of living beings. In humans, it is used to treat schistosomiasis. tapeworm infections, cysticercosis, and more. It works through increasing the permeability of the membranes of schistosome cells towards calcium ions which leads to contractions of muscles and therefore paralysis of the parasite. You can find detailed UV spectra of Praziquantel and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Praziquantel enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Praziquantel can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and Ethanol. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol- 70/30%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution2.06

Description

Class of CompoundsDrug
Analyzing CompoundsPraziquantel

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Praziquantel
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.