HPLC UV Method for Analysis of Natamycin in Yogurt on Primesep 100 Column

HPLC Method for Analysis of Natamycin on Primesep 100 Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Natamycin.

Natamycin, also known as pimaricin, is an antifungal medication with C33H47NO13 molecular formula. It is primarily used to treat fungal infections of eyelids, conjunctiva, and cornea. On occasion, it is also used as a preservative. It prevents fungi growth by binding to ergosterol in fungal cell membranes and inhibiting amino acid and glucose transport proteins. You can find detailed UV spectra of Natamycin and information about its various lambda maxima by visiting the following link.

**The Yogurt sample was prepared by mixing 5g of yogurt and 5g or acetonitrile. After the mixture separated, the liquid half was removed and filtered.

Natamycin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O 10/90 to 80/20% over 20 min
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 320 nm
Limit Of Detection*Natamycin – 0.2 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsDrug
Analyzing CompoundsNatamycin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Natamycin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.