HPLC ELSD Method for Analysis of FMOC Derivatized AMPA and Glyphosate on Chromni Column

HPLC Method for Analysis of Aminomethylphosphonic acid (AMPA), Glyphosate on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Aminomethylphosphonic acid (AMPA), Glyphosate.

Aminomethylphosphonic Acid is an aminophosphonate with the chemical formula CH6NO3P. It is primarily used as a biocide and pesticide, as well as in research to assess the exposure of glyphosate. It is a major toxic metabolite of glyphosate as is considered to be a similar toxicological concern. You can find detailed UV spectra of Aminomethylphosphonic acid (AMPA) and information about its various lambda maxima by visiting the following link.

Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care. You can find detailed UV spectra of Glyphosate and information about its various lambda maxima by visiting the following link.

Aminomethylphosphonic acid (AMPA), Glyphosate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ammonium acetate. Detection is performed using UV.

Condition

ColumnChromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O
BufferAmAc pH 3.0 – 10 mM
Flow Rate0.2 ml/min
DetectionUV 260 nm

Description

Class of CompoundsAminophosphonate & Herbicide
Analyzing CompoundsAminomethylphosphonic acid (AMPA), Glyphosate

Application Column

Chromni

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Aminomethylphosphonic acid (AMPA)
Glyphosate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.