HPLC Chiral Separation of Donepezil Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Donepezil on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Donepezil.

Donepezil is a medication with the chemical formula C24H29NO3. It is primarily used to treat dementia and improve cognition, but has not shown to cause any change in the progression of the disease. Some research suggests that it can be used to treat Lewy body dementia, effects of traumatic brain injury, and vascular dementia. You can find detailed UV spectra of Donepezil and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Donepezil enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Donepezil can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA – 90/10/0.2%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution3.46

Description

Class of CompoundsMedication
Analyzing CompoundsDonepezil

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Donepezil
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.