HPLC Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate on Primesep B Column

HPLC Method for Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate on Primesep B by SIELC Technologies

 

HPLC Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate on Primesep B Column_1474

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate

Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate is an ultra violet hindered amine light stabilizer with the chemical formula C28H52N2O4. It is commonly used in plastics production and in the packaging and manufacturing of pharmaceutical products. It helps prevent oxidation caused by sunlight weathering in coatings and paint. In 2024, it was found added in illicitly sold fentanyl.

Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate can be retained and analyzed on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.

Condition
Column Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description
Class of Compounds
 Hydrophobic
Analyzing Compounds Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate

 

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.