HPLC Chiral Separation of Bifonazole on Cyralis CM Column

HPLC Method for Analysis of Bifonazole on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bifonazole.

Bifonazole is an antifungal drug with the chemical formula C22H18N2. It is primarily sold as an ointment that works through inhibiting fungal ergostrol biosynthesis, which enables it to work against dermatophytes.

This application describes a normal-phase chiral HPLC method for the separation of Bifonazole enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Bifonazole can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA- 85/15/0.5%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution2.32

Description

Class of CompoundsFungicide
Analyzing CompoundsBifonazole

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bifonazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.