HPLC ELSD Method  for Separation of Endocannabinoid-Related Lipids on Chromni Column

HPLC Method for Arachidonoyl ethanolamide (AEA), N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid, N-Arachidonoyl dopamine, N-Arachidonoyl-L-Serine on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Arachidonoyl ethanolamide (AEA), N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid, N-Arachidonoyl dopamine, N-Arachidonoyl-L-Serine.

Arachidonoyl ethanolamide, also denoted as AEA, is a bioactive lipid that has the chemical formula C22H37NO2 and plays significant roles in various physiological and biochemical processes. AEA influences inflammatory and pain pathways due to being an endocannabinoid, meaning it binds to cannabinoid receptors as THC in cannabis does. You can find detailed UV spectra of AEA and information about its various lambda maxima by visiting the following link.

N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid is ab endogenous lipid messenger with the chemical formula C24H39NO4. It occurs naturally in brains of mammals. Due to containing aminobutyric acid, it plays a role in regulating GABAergic pathways. As an endocannabinoid, it also binds to specific g-protein receptors. You can find detailed UV spectra of N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid and information about its various lambda maxima by visiting the following link

N-Arachidonoyl Dopamine, also written as NADA, is an endocannabinoid with the chemical formula C28H41NO3. It acts as an agonist of the CB1 receptor as well as the transient receptor potential V1. You can find detailed UV spectra of NADA and information about its various lambda maxima by visiting the following link.

N-Arachidonoyl-L-Serine, also written as ARA-S, is an a lipid with the chemical formula C23H37NO4. Unlike classic endocannabinoids, it exhibits only a weak affinity for CB1, CB2, and TRPV-1. Instead, it works as an agonist for GPR55 receptor. You can find detailed UV spectra of ARA-S and information about its various lambda maxima by visiting the following link.

Arachidonoyl ethanolamide (AEA), N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid, N-Arachidonoyl dopamine, N-Arachidonoyl-L-Serine can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Acetate buffer. Detection is performed using MS.

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 98/2%
BufferFormic Acid – 0.2%
Flow Rate1.0 mL/min
DetectionELSD
Class of Compounds
Endocannabinoids
Analyzing CompoundsArachidonoyl ethanolamide (AEA), N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid, N-Arachidonoyl dopamine, N-Arachidonoyl-L-Serine

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Arachidonoyl ethanolamide (AEA)
N-Arachidonoyl dopamine
N-Arachidonoyl-3-hydroxy-y-Aminobutyric Acid
N-Arachidonoyl-L-Serine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.