HPLC Method for Perfluorooctanoic acid (PFOA) on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Perfluorooctanoic acid (PFOA).
Pentadecafluorooctanoic acid, also known as C8 due to it’s chemical being C8HF15O2, is an industrial surfactant. It is classified by the IARC as a carcinogen to humans. Due to it not breaking down via natural processes, it is also considered a “forever chemical.” It can easily move through soil and contaminate water, leading up to it building up[ in fish and wildlife. Despite that, it has wide use across industries from upholstery, to floor wax, to fire fighting sealants. PFOA was a key point in a renowned legal case of Robert Bilott against DuPont.
Perfluorooctanoic acid (PFOA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H2SO4 – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Acid, Hydrophobic |
| Analyzing Compounds | Perfluorooctanoic acid (PFOA) |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
