Separation type: Liquid Chromatography Mixed-mode
Octanoic acid (Caprylic acid) is the common name for the eight-carbon straight-chain fatty acid. It can be found in numerous foods such as coconuts, cherries, plums, pineapple sages, black raspberries, and shallots. Octanoic acid is found to be associated with medium-chain acyl-CoA dehydrogenase deficiency, a genetic disorder that affects the metabolism of converting certain fatty acids to energy.
Octanoic acid can be retained on a mixed-mode Primesep 100 column by using an isocratic method consisting of a 50/50% Acetonitrile (ACN) and water mobile phase with a Phosphoric acid (H3PO4) buffer. UV detection at 210 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Hydrophobic, Acid |
| Analyzing Compounds | Octanoic acid (Caprylic acid) |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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