HPLC Chiral Separation of Secnidazole Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Secnidazole Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Secnidazole.

Secnidazole is a nitroimidazole anti-infective drug with the chemical formula C7H11N3O3. It is used primarily to treat bacterial vaginosis and trichomoniasis. You can find detailed UV spectra of Secnidazole and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Secnidazole enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Secnidazole can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of IPA and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, reversed phase
Mobile PhaseIPA/MeCN
Flow Rate1.0 mL/min
DetectionUV 320 nm
Resolution15.3

Description

Class of CompoundsDrug
Analyzing CompoundsSecnidazole

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: reversed phase

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Application Analytes:
Secnidazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.