HPLC Chiral Separation of Miconazole on Cyralis CM Column

HPLC Method for Analysis of Miconazole on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Miconazole.

Miconazole, is a an antifungal agent with the chemical formula C18H14Cl4N2O. It is primarily used as a cream or ointment to treat fungal infections including but not limited to ring worm, pityriasis versicolor, and yeast infections. It works through inhibiting the 14a-sterol demethylasse fungal enzyme, which reduces production of ergosterol. You can find detailed UV spectra of Miconazole and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Miconazole enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Miconazole can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA- 90/10/0.1%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.58

Description

Class of CompoundsFungicide
Analyzing CompoundsMiconazole

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Miconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.