HPLC Chiral Separation of Ketorolac Enantiomers on Cyralis CM Column

HPLC Chiral Method for Analysis of Ketorolac Enantiomers on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketorolac.

Ketorolac is a nonsteroidal anti-inflammatory drug (NSAID) with the chemical formula C15H13NO3. It is used primarily used to treat moderate to severe pain. Due to a high risk of negative effects, including mortal gastrointestinal bleeding, it is only taken for short intervals of time. Despite the adverse effects, it is a widely available and often prescribed in the United States.

This application describes a normal-phase chiral HPLC method for the separation of Ketorolac enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Ketorolac can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/TFA
Flow Rate1.0 mL/min
DetectionUV 320 nm
Resolution1.59

Description

Class of CompoundsDrug
Analyzing CompoundsKetorolac

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Ketorolac
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.