HPLC Chiral Separation of Indapamide on Cyralis CM Column

HPLC Method for Analysis of Indapamide on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Indapamide.

Indapamide is a an medication with the chemical formula C16H16ClN3O3S. It is primarily used to treat hypertension. It is is an indole derivative of chlorosulphonamide and a sulfamyl diuretic with antihypertensive activity. It exerts its diuretic effect by inhibiting reabsorption of sodium and chloride, primarily as a result of action on the cortical diluting segment of the renal distal tubule. You can find detailed UV spectra of Indapamide and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Indapamide enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Indapamide can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA- 70/29/1%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution4.99

Description

Class of CompoundsMedication
Analyzing CompoundsIndapamide

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Indapamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.