HPLC Chiral Separation of Fluoxetine on Nonporous Cyralis Columns

HPLC Chiral Method for Analysis of Fluoxetine Enantiomers on Cyralis Screening Kit Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fluoxetine.

Fluoxetine is an antidepressant in the SSRI class with the chemical formula C17H18FNO. It is used to treat major depressive disorder, anxiety, obsessive-compulsive disorder, panic disorder, and more. It is the eighteenth most prescribed medication in the United States.

This application describes a normal-phase chiral HPLC method for the separation of Fluoxetine enantiomers using SIELC’s Cyralis nonporous chiral columns. The nonporous kit is a collection of AL, CL, AM and CM nonporous phases designed for ultrafast enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis. These nonporous applications are designed to demonstrate baseline resolution of certain racemates even on very short, low-capacity columns.

Fluoxetine can be retained and analyzed using the Cyralis Screening Kit stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of hexane, IPA, and DEA. Detection is performed using UV.

Condition

ColumnCyralis Screening Kit
Mobile PhaseHexane/IPA/DEA – 98/2/0.3%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.52

Description

Class of CompoundsDrug
Analyzing CompoundsFluoxetine

Application Column

Cyralis Screening Kit

Select options
Application Analytes:
Fluoxetine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.