HPLC Chiral Separation of Epoxybergamottin on Cyralis CM Column

HPLC Method for Analysis of Epoxybergamottin on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Epoxybergamottin.

Epoxybergamottin is a furanocoumarin with the chemical formula C21H22O5. It is an inhibitor of the cytochrome P450 enzyme CYP3A4 that is found in citrus peels. You can find detailed UV spectra of Epoxybergamottin and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Epoxybergamottin enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Epoxybergamottin can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane and IPA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA- 70/30%
Flow Rate1.0 mL/min
DetectionUV 250 nm
Resolution2.14

Description

Class of CompoundsFuranocoumarin
Analyzing CompoundsEpoxybergamottin

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Epoxybergamottin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.