| CAS Number | 7003-89-6 |
|---|---|
| Molecular Formula | C5H13ClN+ |
| Molecular Weight | 122.62 |
| InChI Key | JUZXDNPBRPUIOR-UHFFFAOYSA-N |
| LogP | 0.9 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Chlorocholine
January 26, 2026
If you are looking for optimized HPLC method to analyze Chlorocholine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Simultaneous Determination of -Quat Herbicides on Obelisc R Column
September 22, 2022
HPLC Method for Mepiquat, Paraquat, Diquat, Chlorocholine on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mepiquat, Paraquat, Diquat, Chlorocholine.
Chlormequat is a plant growth regulator with the chemical formula C5H13ClN. It works through inhibiting gibberellin biosynthesis, which leads to reduced cell elongation and thicker stalks.
Mepiquat is a plant growth regulator with the chemical formula C7H16N+. It is used primarily to control the growth rate and quality of the yield.
Paraquat is a widely used, non-selective herbicide known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity.
Diquat is an organic dication that is used as a contact herbicide. It has the chemical formula C12H12Br2N2. It is considered moderately toxic and harmful if swallowed, inhaled, or absorbed through skin. It degrades slowly when in nature due to bonding strongly to minerals and organic particles. Due to that, it is no longer approved for use in the European Union.
Mepiquat, Paraquat, Diquat, Chlorocholine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 40% |
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Herbicides |
| Analyzing Compounds | Mepiquat, Paraquat, Diquat, Chlorocholine |
Application Column
Obelisc R
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Diquat
Mepiquat
Paraquat

