SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.


The Cannsep family of columns are a specially-developed line of reverse phase-based columns designed to retain and separate most of the compounds attributed to cannabis physiological properties. Between the three Cannsep columns - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all of the various cannabinoids providing different and significantly orthogonal selectivity.


SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.


The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.


SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.


The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.


The Promix family of mixed-mode columns offer an alternative chromatography technology to deliver efficient resolution of peptides and proteins. The technology is based on a combination of both hydrophobic and ionic interactions. This approach is possible due to a new type of separation media: a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support. With this phase, unparalleled selectivity and peak capacity can be achieved. Independent adjustment of the amount of buffer and organic modifier creates an infinite number of separation conditions that are suitable for many types of bio-molecules.


The SHARC™ family of innovative columns are are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is an interaction (attraction) of bound hydrogen atom to the molecules with electronegative atoms such as oxygen, nitrogen, and fluorine.


SIELC Technologies has developed mixed-mode Columns based on solid-core-porous-shell particles. These specially-developed silica nanoparticles mimic UHPLC conditions on regular HPLC systems, reducing the need for more complicated equipment that typical UHPLC systems require. This separation media combines the efficiency of solid-core technology with the unique selectivity of Ion-Exchange (IE) mode, reverse phase (RP) mode, and HILIC mode. Currently, 3 mixed-mode Primesep columns are available with solid-core, 2.7 µm particles: Primsep 100, Primesep S, and Primesep SB.

Specialty Columns

There are several million compounds isolated and described in chemical literature. An even greater number of combinations of these compounds (mixtures) require analysis by HPLC. SIELC produces custom phases for our customers who are not able to obtain desirable results with commercially available HPLC phases. You can find out more about our special custom LC phases tailored for specific separations by contacting us at