Primesep stationary phases offer unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications.
A wide range of HPLC columns with id’s from 0.5 mm to 22 mm are available. Corresponding Primesep guard columns are available with all
stationary phases and do not require holders. SIELC offers a method development service which is available for all customers.
Ask about our special custom LC-phases tailored for specific separations.

A column with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing
as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is
contained within the column adapter (no external leakage). Read more.

Two complimentary columns, Obelisc R and Obelisc N, based on Liquid Separation Cell ( LiSC ™) technology, offer a new approach to separate
a variety of small molecules. Buffer concentration, buffer pH, and organic modifier concentration are three orthogonal parameters
of the mobile phase that allow adjustment of column properties to separate complex mixtures.

SIELC has developed a special line of Cannsep columns to resolve most of the compounds attributed to cannabis physiological properties.
Three columns: Cannsep A, Cannsep B, and Cannsep C resolve all the cannabinoids providing different and
significantly orthogonal selectivity.

SIELC has developed the Legacy column, which can be used in many USP Validated methods.

Promix is an alternative chromatography technology for efficient resolution of peptides and proteins. The technology is based on a
combination of two interactions – hydrophobic and ionic. This approach is possible due to a new type of separation media: a
chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support.
With this phase, unparalleled selectivity and peak capacity can be achieved. Independent
adjustment of the amount of buffer and organic modifier creates an
infinite number of separation conditions that are suitable
for many types of bio-molecules.

SHARC™ HPLC columns are the latest, innovative columns from SIELC Technologies, the inventors of Primesep®. SHARC™
columns are the first commercially available columns with separation based primarily on hydrogen bonding.
SHARC stands for  Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding is
an interaction (attraction) of bound hydrogen atom to the molecules with electronegative
atoms such as oxygen, nitrogen, and fluorine.

–Specialty Columns
There are several million compounds isolated and described in chemical literature. An even greater number of combinations
of these compounds (mixtures) requires analysis by HPLC. SIELC produces custom phases for our customers who
were not able to obtain desirable results with commercially available HPLC phases.

SIELC Technologies has developed mixed-mode Columns based on solid-core-porous-shell particles. This separation
media combines the efficiency of solid-core technology with the unique selectivity of Ion-Exchange (IE),
reverse phase (RP), surface chemistry and ion-exchange HILIC surface.