BIST™ – a New Mode of LC Separation

Bridge Ion Separation Technology, or BIST™, is a brand new LC separation technique developed by SIELC. The goal of BIST™ is simple – expand the scope of HPLC applications.

SIELC Technologies’ BIST™ is a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! In short, BIST™ is complex chromatography without the hassle.

BIST™ provides an additional separation tool to a whole class of organic and inorganic charged molecules. BIST™ columns, simple mobile phases, and an array of applications provides a whole new continent on the chromatograph
y map.

Analyze Ions Differently

When doubly-charged ions are present in the mobile phase (MP), the surface of the stationary phase can switch its polarity.
For example: if the surface is positively-charged and the doubly-charged ions in the mobile phase are sulfate ions with a
minus 2 charge (from ionized sulfuric acid), then the surface can became negatively-charged.

However, this can occur only when the mobile phase has a relatively low concentration of water. When water is
the main component of the mobile phase, it forms a solvation shell around each ion and prevents the surface
charge from switching.

The same surface switch is observed when a negatively-charged surface interacts with doubly-charged
positive ions such as diamines, and some inorganic ions such as Mg2+ and Ca2+.

BIST™ Products

For more information and a complete look at BIST™, please check out our other BIST™ pages and our brochure below!


BIST™ Brochure
The Theory Behind BIST™
BIST™ Applications

BIST Ionic Modifier Preparation

Copyright  © 2022 by SIELC Technologies Corporation.

The information and disclosures contained herein were originated by and are the property of  SIELC Technologies Corporation and may not be copied, distributed, republished, translated, uploaded, posted, transmitted, stored, or otherwise used in any shape, manner, or form by any means without the specific prior written permission of SIELC Technologies Corporation.  Modification or use of this material for any reason shall be construed as a violation of the property rights of SIELC Technologies Corporation.  SIELC Technologies Corporation reserves all rights, including without limitation all patent, proprietary design, manufacturing, reproduction, use, advertising, and sales rights thereto, and to any articles disclosed herein, except to the extent such rights are granted to others by law or by SIELC Technologies Corporation. BISTTM is a registered trademark of SIELC Technologies Corporation.  U.S. Patents Pending. All data were obtained in SIELC Technologies labs.